Summary: ZnO NPs were successfully synthesized using Tinospora cordifolia stem extract and characterized via UV-Vis, FTIR, SEM, and EDX. The NPs showed a spherical morphology (40 nm) and significant antifungal activity, with 88.24% inhibition at 1000 ppm. The study confirms the effectiveness of plant-mediated green synthesis in producing stable, bioactive nanoparticles, suggesting their potential in antifungal formulations, crop protection, and medical applications while promoting sustainable nanotechnology practices.