Summary: This research isolated and characterized native Trichoderma strains from tomato rhizospheres in Ethiopia to evaluate their antagonistic effects against P. infestans. Ten isolates were obtained, and four strains exhibited substantial growth inhibition of the pathogen, with Ju-TGDb-2 showing the highest efficacy (61.4%). These findings suggest that native Trichoderma isolates can be used as effective biocontrol agents, offering a promising solution for managing late blight in tomatoes.